| 质粒名称: | pegCTNNB1 S45del |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 169844 |
| 存储实验室: | Wen Xue |
|
载体骨架基本信息 |
|
| 载体名称: | pmd127 |
| 载体出品公司: | Clontech |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: |
pegCTNNB1 S45DEL
gRNA/shRNA
|
| 物种来源: | M. musculus (mouse) |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 |
HU6F
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | pegCTNNB1 S45del was a gift from Wen Xue (Addgene plasmid # 169844 ; http://n2t.net/addgene:169844 ; RRID:Addgene_169844) |
| 参考文献部分: | Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice. Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W. Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w. 10.1038/s41467-021-22295-w PubMed 33837189 |
买家导航
pegCTNNB1 S45del载体质粒基本信息
pegCTNNB1 S45del质粒图谱载体图谱和pegCTNNB1 S45del载体序列质粒序列多克隆位点信息
pegCTNNB1 S45del质粒载体简介
pegCTNNB1 S45del质粒序列载体序列
LOCUS pegCTNNB1 S45del 3320 bp DNA circular SYN 26-AUG-2024
DEFINITION pegRNA used for installation (via TCC deletion) of the oncogenic
S45del in Ctnnb1 in mouse liver..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3320)
AUTHORS Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir
A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W
TITLE Improved prime editors enable pathogenic allele correction and
cancer modelling in adult mice.
JOURNAL Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w.
PUBMED 33837189
REFERENCE 2 (bases 1 to 3320)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2021-04-9"; volume: "12(1):2121. doi"; pages: "
10.1038/s41467-021-22295-w"
FEATURES Location/Qualifiers
source 1..3320
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 5..109
/gene="bla"
/label=AmpR promoter
CDS 110..970
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(328..347)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1141..1729
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1630..1649
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1883..1900
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2017..2038
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2053..2083
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2091..2107
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2096..2118
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2115..2131
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2115..2131
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2150..2170
/label=T3
/note="T3 promoter, forward primer"
promoter 2152..2170
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2217..2465
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 2217..2237
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2388..2407
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 2487..2562
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(2616..2632)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2617..2633)
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind complement(2657..2676)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2658..2676)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2686..2703)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2686..2702)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2695..2717)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2844..3299
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2931..2950)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3141..3162
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
61 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
121 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
181 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
241 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
301 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
361 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
421 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
481 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
541 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
601 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
661 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
721 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
781 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
841 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
901 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
961 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
1021 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
1081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
1141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
1201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
1261 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
1321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
1381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
1441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
1501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
1561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
1621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
1681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
1741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
1801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
1861 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
1921 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
1981 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
2041 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
2101 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
2161 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgccccc ttcaccgagg
2221 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
2281 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
2341 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
2401 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
2461 acaccgaggg ttgcccttgc cactcagttt tagagctaga aatagcaagt taaaataagg
2521 ctagtccgtt atcaacttga aaaagtggga ccgagtcggt ccacagctcc tctgagtggc
2581 aagggcaatt tttttgaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
2641 cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
2701 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
2761 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
2821 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
2881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
2941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
3001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
3061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
3121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
3181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
3241 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
3301 ggtggcactt ttcggggaaa
//
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pegCTNNB1 S45del质粒载体应用举例
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